Kelly Lab Protocol Book

0413KellyJCI_release_FINAL_LT revised_5-9-13.docPress Release - Gan et al, JCI 2013
Adenovirus-10-2005.pdfAdenovirus
Adult Primary Mouse Satellite Cell Culture_07June11.pdfAdult Primary Mouse Satellite Cell Culture June 2011
ATPase_muscle_histology2-28-06.pdfATPase Muscle Histology
ChIP_Protocol5-29-07.pdfChIP Protocol
ChIP_Solutions_and_Buffers_5-29-07.pdfChIP Buffers
citrate_synthase_activity.pdfCitrate Synthase Activity
Cloning_Heterologous_Constructs5-27-07.pdfCloning Heterologous Constructs
extraction_of_genomic_DNA_from_tails-9-2005.pdfExtraction of Genomic DNA from tails
fatty_acid_oxidation_in_fibroblasts7-10-06.pdfFatty Acid Oxidation in Fibroblasts
GeneChip Protocol-5-27-07.pdfGeneChip Protocol
Glucose Tolerance Test April 2012.pdfGlucose Tolerance Test April 2012
glucose_oxidation8-30-05.pdfGlucose Oxidation
Glut_1-4_Westerns6-27-07.pdfGLUT 1-4 Westerns
Glycogen_assay1-4-2007.pdfGlycogen Assay
glycogen_data_blank_template.xlsGlycogen Data, Blank Template
immunohistochemistry_for_PPAR.pdfImmunohistochemistry for PPARalpha
immunoprecipitation9-05.pdfImmunoprecipitation
Insulin_Tolerance_Test1-4-2007.pdfInsulin Tolerance Test
IRS_Immunoprecipitation1-4-2007.pdfIRS Immunoprecipitation
lactate_measurements_from_mouse_tail.pdfLactate Measurements from Mouse Tail
Ligation_of_cDNA_into_Vector1-5-07.pdfLigation of cDNA into Vector (Subcloning)
mitochondrial_isolation_protocol-heart09-14-05.pdfMitochondrial Isolation (Heart)
mitochondrial_isolation_skeletal_muscle7-14-06.pdfMitochondrial Isolation from Skeletal Muscle
mtDNAquantification-cells_or_tissue7-14-06.pdfDNA Extraction from Cells or Tissue for mtDNA Quantification
perfusion_fixation_of_whole_mouse7-15-06.pdfPerfusion Fixation of Whole Mouse
pierce_protein_determination.pdfPierce Protein Determination
preparation_of_membrane_proteins_from_tissue.pdfPreparation of Membrane Proteins from Human Tissue Samples
Preparation_of_nuclear_proteins_from_tissue.pdfPreparation of Nuclear Proteins from Tissue
primary_mouse_fibroblasts_from_tail.pdfMurine Fibroblasts (from tail clip)
QIAGEN_plasmid_maxiprep_with_troubleshoot7-15-06.pdfQIAGEN Plasmid Maxi Prep
Rat Neonatal Cardiocyte April 2011.pdfRat Neonatal Cardiocyte April 2011
reporter_assay-dualglo10-15-2005.pdfReporter Assay using Dual-Glo
respiration_buffers_protocol4-26-05.pdfRespiration Buffers for Skinned Fibers
respiration_protocol.pdfMitochondrial Respiration - Skinned Fiber
RNA_and_northern_blot3-3-2006.pdfRNA Preparation and Northern Blot
RNA_from_cells11-2005.pdfRNA Preparation from Cells in Culture
SDH_muscle_histology2-21-06.pdfSDH Muscle Histology
Sequencing Protocol6-27-07.pdfSequencing
southern2-24-05.pdfSouthern (DNA)
STORM10-2005.pdfSTORM Phosphorimager
Taqman8-7-07.pdfTAQMAN
Tissue_Prep_for_SDHATPase stain-CZ-6-27-07.pdfTissue Prep for SDH and ATPase Stain
Transgenic_mice_Generation6-28-07.pdfTransgenic Mice Generation
Transient_Transfection12-4-07.pdfTransient Transfections
Western_protocol7-7-06.pdfWestern Protocol
whole_cell_extracts-cells7-15-06.pdfWhole Cell Extracts (Cells)
whole_cell_extracts-PGC.pdfWhole Cell Protein Prep
whole_cell_extracts-tissue7-15-06.pdfWhole Cell Extracts (Tissue)